RNA

Part:BBa_K1614011:Design

Designed by: Frieda Anna Sorgenfrei   Group: iGEM15_Heidelberg   (2015-09-16)


Spinach in BBF RFC 110 transcription cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 113
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 113
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 113
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 113
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 155
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cloned without restriction enzymes to avoid issues occurring from conserved cut sites. For more details see RFC 110.


Source

Synthetic (Babendure, J.R., Adams, S.R., and Tsien, R.Y. (2003). Aptamers switch on fluorescence of triphenylmethane dyes. Journal of the American Chemical Society 125, 14716-14717.)


References